论文题目：Plasma membrane-associated calcium signaling modulates zinc homeostasis in Arabidopsis
论文作者：Yanjun Fang#, Chuanfeng Ju#，Laiba Javed#，Chenyu Cao，Yuan Deng, Yaqi Gao, Xuanyi Chen，Lv Sun, Yusheng Zhao, Cun Wang*
论文摘要：Zinc (Zn) is a crucial micronutrient for all organisms, and its deficiency can significantly hamper crop yield and quality. However, the understanding of the regulatory mechanisms involved in plant Zn signal perception and transduction remains limited. In this study, we discovered that the Ca2+-CBL1/4/5/8/9-CIPK3/9/23/26-ZIP12 signaling module effectively responds to Zn deficiency and regulates Zn homeostasis in Arabidopsis thaliana . Furthermore, we determined that CIPK3/9/23/26 interact with the Zn transporter ZIP12 and phosphorylate it primarily at Ser185. This phosphorylation event was crucial for the stability of the ZIP12 protein, suggesting that it regulates the function of ZIP12 in Zn transport. Collectively, our findings identify a plasma membrane-associated calcium signaling pathway that regulates Zn homeostasis in Arabidopsis thaliana . This pathway represents a promising target for molecular breeding approaches aimed at developing crops with enhanced tolerance to Zn deficiency.
锌（Zn）作为所有生物不可或缺的微量营养元素，其缺乏会显著影响作物的产量和品质。尽管如此，关于植物如何感知并传导锌信号的调控机制，目前的研究仍很欠缺。本研究揭示了一个新发现：低锌胁迫能够触发拟南芥根部产生明显的Ca2+信号。这一过程中，定位于质膜的Ca2+感受器CBL1/4/5/8/9及其相互作用的蛋白激酶CIPK3/9/23/26响应低锌胁迫。进一步研究表明，CIPK3/9/23/26与锌转运蛋白ZIP12具有强烈的相互作用，并磷酸化修饰ZIP12的第185位丝氨酸残基。通过一系列生理生化实验，我们进一步确认，第185位丝氨酸对ZIP12的蛋白稳定性具有重要作用。综上所述，本研究不仅阐明了Ca2+-CBL1/4/5/8/9-CIPK3/9/23/26-ZIP12信号通路在维持植物锌稳态中的关键作用，而且为作物锌营养高效利用的遗传改良提供了分子靶标。
论文链接：https://doi.org/10.1016/j.scib.2025.02.010.