张新梅等《Horticulture Research》2023年

论文题目：CaREM1.4 interacts with CaRIN4 to regulate Ralstonia solanacearum tolerance by triggering cell death in pepper

论文作者：Yanqin Zhang, Shuangyuan Guo, Feng Zhang, Pengfei Gan, Min Li, Cong Wang, Huankun Li, Gang Gao, Xiaojie Wang, Zhensheng Kang, Xinmei Zhang

论文摘要：

Remorins (REMs), plant-specific proteins,act a significant role in conferring plants the ability to adapt to adverse environments. However, the accurate function of REMs in resistance to biological stress remains largely unknown. 18 CaREM genes were identified in the pepper genome sequences based on the C-terminal conserved domain that is specific to remorin proteins in this research. Phylogenetic relations, chromosomal localization, motif, gene structures, and promoter regions of these REMs were analyzed and a remorin gene, CaREM1.4, was cloned for further study. The transcription of CaREM1.4 in pepper was induced by infection with Ralstonia solanacearum. Knocking down CaREM1.4 in pepper using virus-induced gene silencing (VIGS)technologies reduced the resistance of pepper plants to R. solanacearum and down-regulated the expression of immunity-associated genes. Conversely, Transient over-expression of CaREM1.4 in pepper and Nicotiana benthamiana plants triggered hypersensitive response (HR)-mediated cell death and up-regulated expression of defense-related genes. In addition, CaRIN4-12, which interacted with CaREM1.4 at the plasma membrane and cell nucleus, was knocked down with VIGS, decreasing the susceptibility of C. annuum to R. solanacearum. Furthermore, CaREM1.4 reduced ROS production by interacting with CaRIN4-12 upon co-injection inpepper. Taken together, our findings suggest that CaREM1.4 may function as a positive regulator of HR, and it interacts with CaRIN4-12 which negatively regulates plant immune responsesduring the pepper to R. solanacearum.Our study provides new evidence for comprehending the molecular regulatory network of plant cell death.

Remorins（REMs）作为一种植物特异性蛋白，在植物防御反应中发挥着重要功能。然而，大多数Remorin基因在应对生物胁迫中的确切功能仍有待进一步研究。本研究根据Remorin蛋白的C端保守结构域鉴定了18个辣椒的CaREMs基因，对这些CaREMs基因进行了系统发育分析、染色体定位、保守基序及基因结构等分析，并成功克隆了一个remorin基因CaREM1.4进行进一步研究。CaREM1.4在青枯菌处理后显著上调表达，使用病毒诱导基因沉默（VIGS）技术敲除CaREM1.4降低了辣椒植物对青枯菌的抗性，并下调了免疫相关基因的表达。相反，在辣椒和本氏烟草中的瞬时过表达CaREM1.4能够产生超敏反应，触发细胞死亡，并上调免疫相关基因的表达。此外，CaREM1.4能够与CaRIN4-12在细胞膜和细胞核上存在互作关系，利用VIGS技术敲除CaRIN4-12能显著降低辣椒对青枯菌的易感性。进一步研究发现CaRIN4-12能够抑制由CaREM1.4引起的ROS迸发及细胞坏死，这些结果表明，CaREM1.4为辣椒与青枯菌互作的正调控因子，而CaRIN4-12通过与前者相互作用并影响其功能，发挥负调控作用。我们的研究为理解植物细胞死亡的分子调控网络提供了新的证据。

论文链接：https://doi.org/10.1093/hr/uhad053